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vector red chromogen substrate  (Vector Laboratories)


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    Structured Review

    Vector Laboratories vector red chromogen substrate
    Vector Red Chromogen Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1926 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+red+chromogen/VECTOR+Red+Alkaline+Phosphatase+(Red+AP)+Substrate+Kit/pmc12628778-299-51-55
    Average 96 stars, based on 1926 article reviews
    vector red chromogen substrate - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Staining:

    Article Title: Characterization of serum and tissue oxytocinase and tissue oxytocin in the pregnant and non-pregnant mare
    Article Snippet: After that, the membranes were washed in PBS containing 0.05% tween 20 for 5 min for 3 times, and incubated with biotinylated goat anti-rabbit IgG (Vector Laboratories, Burlingame, CA, USA, 1:1,000 in PBS) for 30 min and followed by incubation with streptavidin conjugated to alkaline phosphatase (Vector Laboratories, Burlingame, CA, USA, 1:500 in PBS) for 20 min at room temperature. .. The positive staining was visualized by incubation with ImmPACT ® Vector ® Red chromogen (Vector Laboratories, Burlingame, CA, USA). .. Precision Plus ProteinTM Dual Color Standards (Bio-Rad, Hercules, CA, USA) were used for monitoring gel electrophoresis and confirmation of proper protein transfer.

    Article Title: The wake and sleep-modulating neurons of the lateral hypothalamic area demonstrate a differential pattern of degeneration in Alzheimer’s disease
    Article Snippet: Following overnight primary antibody incubation, the sections were incubated in secondary biotinylated anti-rabbit IgG (1:400, Cat# BA-1100, Vector Labs, CA, USA) and secondary conjugated-HRP anti-mouse IgG (1:400, cat# R-05071-500, Advansta, CA, USA) for 1.5 hours at room temperature. .. The stained sections were then developed using immPACT DAB Peroxidase (HRP) Substrate Kit (Cat# SK-4150, Vector Labs, CA, USA), Vectastain ABC-AP kit (Cat#AK-5000, Vector Labs, CA, USA), and Vector Red chromogen (Cat# SK-5105, Vector Lab, CA, USA). .. To estimate the total number of neurons, all sections were counterstained using gallocyanin (Cat# A12936, Alfa Aesar, MA, USA) as a nucleic acid stain at a previously optimized pH (pH 1.9 - 2.1).

    Article Title: Partial Rescue of Ocular Pigment Cells and Structure by Inducible Ectopic Expression of Mitf-M in MITF-Deficient Mice
    Article Snippet: After addition of a nonspecific protein blocker (X0909; Dako), slides were stained with a Dct antibody (Pep8h, gift from Vincent Hearing, National Cancer Institute) by incubation at a 1:5000 dilution overnight at 4°C. .. The staining was revealed using ImmPRESS anti-rabbit alkaline phosphatase polymer (Vector Laboratories, Burlingame, CA, USA), developed with Vector Red chromogen (Vector Laboratories) followed by counterstaining with Mayer's hematoxylin (#MHS32; Sigma Aldrich, St. Louis, MO, USA) before coverslipping. ..

    Incubation:

    Article Title: Characterization of serum and tissue oxytocinase and tissue oxytocin in the pregnant and non-pregnant mare
    Article Snippet: After that, the membranes were washed in PBS containing 0.05% tween 20 for 5 min for 3 times, and incubated with biotinylated goat anti-rabbit IgG (Vector Laboratories, Burlingame, CA, USA, 1:1,000 in PBS) for 30 min and followed by incubation with streptavidin conjugated to alkaline phosphatase (Vector Laboratories, Burlingame, CA, USA, 1:500 in PBS) for 20 min at room temperature. .. The positive staining was visualized by incubation with ImmPACT ® Vector ® Red chromogen (Vector Laboratories, Burlingame, CA, USA). .. Precision Plus ProteinTM Dual Color Standards (Bio-Rad, Hercules, CA, USA) were used for monitoring gel electrophoresis and confirmation of proper protein transfer.

    Article Title: In situ staining with antibodies cross-reactive in pigs, cattle, and white-tailed deer facilitates understanding of biological tissue status and immunopathology.
    Article Snippet: Identifying cellular markers within archived formalin-fixed, paraffin-embedded (FFPE) tissues is critical for understanding tissue landscapes impacting animal health, but in situ detection methods are limited in veterinary species by a restricted toolbox of species-compatible immunoreagents.. We identify antibodies with conserved in situ reactivity to IBA-1 (macrophages/dendritic cells), CD3ε (T cells), Pax5 (B cells), Ki-67 (cycling cells), and cytokeratin type I/II (epithelial cells) in FFPE tissues of pigs, cattle, and white-tailed deer.. Multiplexed brightfield detection (IBA-1/CD3ε/Pax5) in lymph nodes of all three species demonstrated species-specific and speciesconserved features of cellular architecture.

    Polymer:

    Article Title: Partial Rescue of Ocular Pigment Cells and Structure by Inducible Ectopic Expression of Mitf-M in MITF-Deficient Mice
    Article Snippet: After addition of a nonspecific protein blocker (X0909; Dako), slides were stained with a Dct antibody (Pep8h, gift from Vincent Hearing, National Cancer Institute) by incubation at a 1:5000 dilution overnight at 4°C. .. The staining was revealed using ImmPRESS anti-rabbit alkaline phosphatase polymer (Vector Laboratories, Burlingame, CA, USA), developed with Vector Red chromogen (Vector Laboratories) followed by counterstaining with Mayer's hematoxylin (#MHS32; Sigma Aldrich, St. Louis, MO, USA) before coverslipping. ..

    Article Title: Pericytic mimicry in well-differentiated liposarcoma / atypical lipomatous tumor
    Article Snippet: Immunohistochemistry for pericyte markers was performed using the ABC method (Vectastain Elite ABC, Vector Laboratories, Burlingame, CA, USA) using DAB as the chromogen (ImmPACT DAB, Vector Laboratories). .. Multiple antigens were detected by multiplexing the ABC method and DAB chromogen with an alkaline phosphatase polymer detection method (ImmPress-AP Polymer Detection, Anti-mouse IG, Vector Laboratories) and Vector Red® chromogen (Vector Red® Alkaline Phosphatase Substrate, Vector Laboratories). .. The following primary antibodies were used: monoclonal mouse anti-MDM2 (1/100, EMD Millipore), monoclonal mouse anti-αSMA (1/75, [1A4], ABCAM), monoclonal rabbit anti-CD146 (1/500, EPR3208, ABCAM, Cambridge, MA, USA), monoclonal rabbit anti-PDGFRβ (1/100, [2E8E1], monoclonal mouse anti-RGS5 (1/100, [89C2], Cell Signaling Technologies), and monoclonal mouse anti-CD31 (1/100, [89C2], Cell Signaling Technologies).

    Multiplexing:

    Article Title: Pericytic mimicry in well-differentiated liposarcoma / atypical lipomatous tumor
    Article Snippet: Immunohistochemistry for pericyte markers was performed using the ABC method (Vectastain Elite ABC, Vector Laboratories, Burlingame, CA, USA) using DAB as the chromogen (ImmPACT DAB, Vector Laboratories). .. Multiple antigens were detected by multiplexing the ABC method and DAB chromogen with an alkaline phosphatase polymer detection method (ImmPress-AP Polymer Detection, Anti-mouse IG, Vector Laboratories) and Vector Red® chromogen (Vector Red® Alkaline Phosphatase Substrate, Vector Laboratories). .. The following primary antibodies were used: monoclonal mouse anti-MDM2 (1/100, EMD Millipore), monoclonal mouse anti-αSMA (1/75, [1A4], ABCAM), monoclonal rabbit anti-CD146 (1/500, EPR3208, ABCAM, Cambridge, MA, USA), monoclonal rabbit anti-PDGFRβ (1/100, [2E8E1], monoclonal mouse anti-RGS5 (1/100, [89C2], Cell Signaling Technologies), and monoclonal mouse anti-CD31 (1/100, [89C2], Cell Signaling Technologies).

    Avidin-Biotin Assay:

    Article Title: Airway Hyperresponsiveness, Inflammation, and Pulmonary Emphysema in Rodent Models Designed to Mimic Exposure to Fuel Oil–Derived Volatile Organic Compounds Encountered during an Experimental Oil Spill
    Article Snippet: CD31 detection was developed with Vector Novared chromogen, and neutrophil elastase with diaminobenzidine (DAB)–nickel (Vector Laboratories) on respective slide sets. .. The anti-VEGF and anti-CD8 primary antibodies were followed by a ) biotinylated goat anti-rabbit IgG and horse anti-mouse IgG secondary antibodies, respectively (both from Vector Laboratories) at 1:200 dilution ( 7.5 μ g / mL ); b ) an alkaline phosphatase avidin–biotin complex (ABC-AP, Vector Laboratories); and c ) Vector® Red chromogen (Vector Laboratories). .. All second-step reagents (ImmPRESS® reagent and biotinylated secondary antibodies) were adsorbed in 10% normal rat serum (STEMCELL Technologies).



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    Vector Laboratories chromogenic alkaline phosphatase solution
    Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by <t>chromogenic</t> <t>alkaline</t> <t>phosphatase</t> (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).
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    Vector Laboratories vector red alkaline phosphatase chromogenic substrate
    Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by <t>chromogenic</t> <t>alkaline</t> <t>phosphatase</t> (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).
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    Image Search Results


    Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by chromogenic alkaline phosphatase (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).

    Journal: Theranostics

    Article Title: Superior Performance of Aptamer in Tumor Penetration over Antibody: Implication of Aptamer-Based Theranostics in Solid Tumors

    doi: 10.7150/thno.11711

    Figure Lengend Snippet: Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by chromogenic alkaline phosphatase (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).

    Article Snippet: The chromogenic alkaline phosphatase solution (Vector Labs, Cat #SK-5100) was prepared according to the manufacturer's instruction and added to the slides following 1 h staining of secondary anti-biotin antibody (Vector Labs, Cat #SP-3020) (1: 250 dilution).

    Techniques: Double Staining, Staining, Injection