vector red chromogen substrate (Vector Laboratories)
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Vector Red Chromogen Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1926 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vector+red+chromogen/VECTOR+Red+Alkaline+Phosphatase+(Red+AP)+Substrate+Kit/pmc12628778-299-51-55
Average 96 stars, based on 1926 article reviews
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Staining:Article Title: Characterization of serum and tissue oxytocinase and tissue oxytocin in the pregnant and non-pregnant mare Article Snippet: After that, the membranes were washed in PBS containing 0.05% tween 20 for 5 min for 3 times, and incubated with biotinylated goat anti-rabbit IgG (Vector Laboratories, Burlingame, CA, USA, 1:1,000 in PBS) for 30 min and followed by incubation with streptavidin conjugated to alkaline phosphatase (Vector Laboratories, Burlingame, CA, USA, 1:500 in PBS) for 20 min at room temperature. .. The positive staining was visualized by incubation with ImmPACT Article Title: The wake and sleep-modulating neurons of the lateral hypothalamic area demonstrate a differential pattern of degeneration in Alzheimer’s disease Article Snippet: Following overnight primary antibody incubation, the sections were incubated in secondary biotinylated anti-rabbit IgG (1:400, Cat# BA-1100, Vector Labs, CA, USA) and secondary conjugated-HRP anti-mouse IgG (1:400, cat# R-05071-500, Advansta, CA, USA) for 1.5 hours at room temperature. .. The stained sections were then developed using immPACT DAB Peroxidase (HRP) Substrate Kit (Cat# SK-4150, Vector Labs, CA, USA), Vectastain ABC-AP kit (Cat#AK-5000, Vector Labs, CA, USA), and Article Title: Partial Rescue of Ocular Pigment Cells and Structure by Inducible Ectopic Expression of Mitf-M in MITF-Deficient Mice Article Snippet: After addition of a nonspecific protein blocker (X0909; Dako), slides were stained with a Dct antibody (Pep8h, gift from Vincent Hearing, National Cancer Institute) by incubation at a 1:5000 dilution overnight at 4°C. .. The staining was revealed using ImmPRESS anti-rabbit alkaline phosphatase polymer (Vector Laboratories, Burlingame, CA, USA), developed with Incubation:Article Title: Characterization of serum and tissue oxytocinase and tissue oxytocin in the pregnant and non-pregnant mare Article Snippet: After that, the membranes were washed in PBS containing 0.05% tween 20 for 5 min for 3 times, and incubated with biotinylated goat anti-rabbit IgG (Vector Laboratories, Burlingame, CA, USA, 1:1,000 in PBS) for 30 min and followed by incubation with streptavidin conjugated to alkaline phosphatase (Vector Laboratories, Burlingame, CA, USA, 1:500 in PBS) for 20 min at room temperature. .. The positive staining was visualized by incubation with ImmPACT Article Title: In situ staining with antibodies cross-reactive in pigs, cattle, and white-tailed deer facilitates understanding of biological tissue status and immunopathology. Article Snippet: Identifying cellular markers within archived formalin-fixed, paraffin-embedded (FFPE) tissues is critical for understanding tissue landscapes impacting animal health, but in situ detection methods are limited in veterinary species by a restricted toolbox of species-compatible immunoreagents.. We identify antibodies with conserved in situ reactivity to IBA-1 (macrophages/dendritic cells), CD3ε (T cells), Pax5 (B cells), Ki-67 (cycling cells), and cytokeratin type I/II (epithelial cells) in FFPE tissues of pigs, cattle, and white-tailed deer.. Multiplexed brightfield detection (IBA-1/CD3ε/Pax5) in lymph nodes of all three species demonstrated species-specific and speciesconserved features of cellular architecture. Polymer:Article Title: Partial Rescue of Ocular Pigment Cells and Structure by Inducible Ectopic Expression of Mitf-M in MITF-Deficient Mice Article Snippet: After addition of a nonspecific protein blocker (X0909; Dako), slides were stained with a Dct antibody (Pep8h, gift from Vincent Hearing, National Cancer Institute) by incubation at a 1:5000 dilution overnight at 4°C. .. The staining was revealed using ImmPRESS anti-rabbit alkaline phosphatase polymer (Vector Laboratories, Burlingame, CA, USA), developed with Article Title: Pericytic mimicry in well-differentiated liposarcoma / atypical lipomatous tumor Article Snippet: Immunohistochemistry for pericyte markers was performed using the ABC method (Vectastain Elite ABC, Vector Laboratories, Burlingame, CA, USA) using DAB as the chromogen (ImmPACT DAB, Vector Laboratories). .. Multiple antigens were detected by multiplexing the ABC method and DAB chromogen with an alkaline phosphatase polymer detection method (ImmPress-AP Polymer Detection, Anti-mouse IG, Vector Laboratories) and Multiplexing:Article Title: Pericytic mimicry in well-differentiated liposarcoma / atypical lipomatous tumor Article Snippet: Immunohistochemistry for pericyte markers was performed using the ABC method (Vectastain Elite ABC, Vector Laboratories, Burlingame, CA, USA) using DAB as the chromogen (ImmPACT DAB, Vector Laboratories). .. Multiple antigens were detected by multiplexing the ABC method and DAB chromogen with an alkaline phosphatase polymer detection method (ImmPress-AP Polymer Detection, Anti-mouse IG, Vector Laboratories) and Avidin-Biotin Assay:Article Title: Airway Hyperresponsiveness, Inflammation, and Pulmonary Emphysema in Rodent Models Designed to Mimic Exposure to Fuel Oil–Derived Volatile Organic Compounds Encountered during an Experimental Oil Spill Article Snippet: CD31 detection was developed with Vector Novared chromogen, and neutrophil elastase with diaminobenzidine (DAB)–nickel (Vector Laboratories) on respective slide sets. .. The anti-VEGF and anti-CD8 primary antibodies were followed by a ) biotinylated goat anti-rabbit IgG and horse anti-mouse IgG secondary antibodies, respectively (both from Vector Laboratories) at 1:200 dilution ( 7.5 μ g / mL ); b ) an alkaline phosphatase avidin–biotin complex (ABC-AP, Vector Laboratories); and c ) |
